Expression of galectin 9 mRNA in lactose maldigestion and children’s obesity

Aleksandr Abaturov, Anna Nikulina

Abstract


OBJECTIVES AND STUDY: To investigate the association of mRNA expression of galectin - 9 (Gal - 9) and lactose maldigestion in children’s obesity with different genotypes of the lactase gene (LCT).

METHODS: The study involved 85 children with obesity (BMI>97th percentile) aged 6-18 years. The study defined genotypes of LCT (material for investigation - venous blood), included expression of Gal - 9 mRNA (material for investigation - buccal epithelium) by real-time polymerase chain reaction analysis, and utilized the study of lactose maldigestion by Hydrogen breath testing. The first group of observations was presented by 44 children with genotype C/C -13910, the second group consisted of 41 children with phenotypically identical genotypes C/T -13910 and T/T -13910, p>0.05.

RESULTS: Lactose maldigestion in children with genotype C/C -13910 averaged 36.05±4.73 ppm, in children with genotypes C/T -13910 - 22.61±4.1 ppm (p<0.05) and with genotype T/T -13910 - was absent (p<0.05). The average expression level of Gal - 9 mRNA in children with genotype C/C -13910 was 564.6±35.8 relation units (RU) ∆mRNA Gal - 9/mRNA actin and was 61.02±15.8 RU ∆mRNA Gal - 9/mRNA actin, p<0.01 in children with genotypes C/T and T/T -13910. The lowest mean level of expression of Gal - 9 mRNA (42.47±13.4 RU ∆mRNA Gal - 9/mRNA actin) was recorded in the subgroup of children with genotype C/C -13910 and lactose maldigestion (n=22). Whereas the largest mean level of expression of Gal - 9 mRNA was recorded in the subgroup of children with the C/C -13910 and without lactose maldigestion (n=22) - 1086.73±51.2 relation units ∆mRNA Gal - 9/mRNA actin, p<0.01.

CONCLUSION: The expression level of Gal - 9 mRNA depends on lactose maldigestion in children with genotype C/C -13910

Keywords


galectin - 9, obesity, children, maldigestion of lactose, lactase

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DOI: http://dx.doi.org/10.36013%2Fijipem.v4i1.52

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